FAQ

Sex Sorted Semen is the 'gender selected' semen used of Artificial Insemination (AI) for cattle and buffaloes to produce more than 90% selected gender (females) whereas conventional semen produces equal proportion of male and female (50:50) calves.

Sex sorting of sperms are done based on the difference in size of the DNA content of X chromosome bearing sperms (female) and Y chromosome bearing sperms (male). SexedULTRA^® sperm sorting technology identifies the difference between X and Y, separate and remove the unwanted sperms without killing. The difference in size of the DNA content of X chromosome bearing sperms is about 3.8% larger in bovines.

SexedULTRA^® sperm sorting technology separates female (X) and male (Y) chromosome bearing sperms and removes the Y without killing. The technology also remove the dead and dying sperms from the raw ejaculate prior to the sorting process. Absence of dead sperms and debris eliminate the toxic effects of dead sperms on live sperms, increases the sperm viability, progressive motility and improve the conception rate significantly. SexedULTRA^® also provide gender purity of more than 90% which ensure the gender ratio of calves born to be more than 90% females. The conception rate of SexedULTRA^® Sex Sorted Semen is proven to be similar to or more than that of conventional semen and the gender ratio of calves born is over 90% in the field.

The average conception rates in cattle and buffalo is proven to be similar or more than that of conventional semen under similar conditions

No, the standard operating protocols for semen handling and artificial insemination (AI Protocol) are the same, but it must be followed strictly for every insemination

SexedULTRA^® Sex Sorted Semen from all breeds of cattle including indigenous, exotic, cross breeds and buffaloes such as Murrah, Jaffrabadi, Mehsana and Pandharpuri are available from our partners such as BAIF, ULDB & MPLPDC

It is recommended to use Sex Sorted Semen on animals that are healthy and do not have any reproductive problems. It can be used for cows and heifers of cattle and buffaloes. The conception rate of SexedULTRA^® Sex Sorted Semen is proven to be similar to or more than that of conventional semen under similar conditions. In general, it is advised to use Sex Sorted Semen from bulls of superior genetics.

SexedULTRA^® Sex Sorted Semen reduces the number of male calves born to be less than 10%. This can reduce the number of male calves born.

It is recommended to inseminate (AI) after 18 hours from the first signs of standing heat. Inseminate animals that show clear signs of good heat and do not have any reproductive problem. (AI Protocol)

SexedULTRA^® Sex Sorted Semen needs to be thawed using temperature regulated thawing units and utilizing a thermometer to monitor temperature. Water temperature should be between 95°F and 98°F (35°C and 37°C, respectively). The thawing process should be at least 60 seconds (1 min.) for cattle, 120 seconds (2 min.) for buffalos and not more than 15 minutes. (AI Protocol)

Female gender purity over 90%. (Average female purity of SexedULTRA^® Sex Sorted Semen in India is 93% which ensures gender ratio calves born to be similar in the field)

Post thaw progressive motility at 0hr is over 50% and 3hr is over 30%. (Average post thaw progressive motility of SexedULTRA^® Sex Sorted Semen in India is 61% at 0hr and 54% at 3hrs post thaw which ensures high conception rate)

Acrosome Integrity at 3hrs post thaw is over 50%. (Average intact acrosomes of SexedULTRA^® Sex Sorted Semen in India is 76% at 3hrs post thaw which ensure high conception rate)

Bacterial count: less than 100 cfu (The average bacterial count in India is zero)

Progressive motility is the percentage of progressively motile sperms and it represents the % of progressively motile sperms in the straw. 0hr post thaw progressive motility is measured just after thawing the straw and the 3hrs post thaw progressive motility is measured after 3hrs incubation at 35 to 36 Degree Celsius. 3hr post thaw incubation progressive motility is very important because it shows the survivability of sperms in the uterus of the cows after AI prior to fertilization. Sperms with high 3hr post thaw progressive motility will have higher chances making the cow or heifer pregnant.

• The average post thaw progressive motility of SexedULTRA^® Sex Sorted Semen in India is averaging 61% at 0hr and 54% at 3hrs post thaw which is 11% and 24% higher than the standard requirement of 50% and 30% respectively

Percentage of Acrosome Integrity is the measure of intact acrosomes, which is measured as a percentage of the total number of sperms in the straw. Intact acrosomes are necessary for the sperms to penetrate the ovum and therefore, higher percentage of intact acrosome will help improving conception rate.

• Average acrosome integrity of SexedULTRA^® Sex Sorted Semen in India is 76% at 3hrs post thaw whereas the standard requirement is only 50% at 0hrs

SexedULTRA^® sperm sorting technology is able to produce Sex Sorted Semen selected for Female or Male because the technology is only separating the female and male sperms without killing the unwanted. Therefore, it is possible to produce male or female Sex Sorted Semen from the same ejaculate simultaneously or separately

Yes, SexedULTRA^® Sex Sorted Semen can be used on cows of any lactations if she is healthy and do not have any reproductive problems. In other words, if an animal can become pregnant from conventional semen, she can also become pregnant from SexedULTRA^® Sex Sorted Semen under similar conditions

As in case of conventional semen, Sex Sorted Semen also do not have any expiry period as long as it is stored safely in sufficient quantity of liquid nitrogen

ST India follows the Govt of India Minimum Standard Protocol (MSP) approved 'plug colours' for different breeds of cattle and buffalos with white/transperent straws as below:

Each straw of SexedULTRA^® Sex Sorted Semen is printed with specific information as below:

• ST - Sexing Technologies (company name)
• SexedULTRA^® - Brand Name
• xF90% - Sex Sorted with 90% Female purity
• LOGO - Logo of ULDB (Organisation to which the bull belongs)
• ULD-JY-105 - Bull's INAPH Id
• JY - Jersey (Breed of the bull)
• ULDB - Organisation to which the bull belongs and location of the laboratory
• 287/2022 - Julian Date on which ejaculate was collected and batch number
• ISO9001:2015 - ISO Certification details
• 11.3K ET - 11300 kg in one lactation (Bull Dam's yield) and ET means Embryo Transfer (source of bull)
• PHC6RB - Unique alpha numeric code

SexedULTRA^® Sex Sorted Semen is available in most of the states in India through the distributors of BAIF, ULDB and MPLPDC. The details can be found at Distributor Page. Where there are no distributors, please contact the above organisations directly BAIF / ULDB / MPLPDC .

Sex Sorted Semen in general is a gender selected semen that is capable of producing more calves of preferred gender. SexedULTRA^® is a patented technology of ST producing high fertility Sex Sorted Semen that ensure more than 90% female calves. In order to get maximum benefits of using Sex Sorted Semen, it is advised to:

• Select your superior cows (better genetics) for use of Sex Sorted Semen because you want a female calf from a good cow
• Please make sure that the cows selected for insemination with Sex Sorted Semen are in good condition, good general and reproductive health without any history of repeat breeding so that the chances of conception will be greater
• Select the Sex Sorted Semen from superior bulls (better genetics) because you will get a female calf from a better bull which will be more productive
• Please follow the semen handling and AI protocol completely and correctly every time AI Protocol
• Keep records such as date of AI, name of the bull used and preferably keep the empty straw until the calf is born to ensure traceability and verification if needed
• For more information, please contact ST India